This LNCS conference volume constitutes the proceedings of the MICCAI Workshop GRAIL 2023 and MICCAI Challenge OCELOT 2023, Held in Conjunction with MICCAI 2023, Vancouver, BC, Canada, September 23, and October 4, 2023. The 9 full papers (GRAIL 2023) and 6 full papers (OCELOT 2023) included in this volume were carefully reviewed and selected from GRAIL 14 (GRAIL 2023) and 6 (OCELOT 2023) submissions. The conference GRAIL 2023 a wide set of methods and application and OCELOT 2023 focuses on the cover a wide range of methods utilizing tissue information for better cell detection, in the sense of training strategy, model architecture, and especially how to model cell-tissue relationships.