The book Applied Artificial Intelligence 2: Medicine, Biology, Chemistry, Financial, Games, Engineering is providing exceptional chapters of the state-of-the-art research knowledge and results on the innovative theories, methodology and applications of artificial intelligence and its sub-domain like deep learning, machine learning in different areas such as medicine, economy, education, law, smart city, government, industry etc. Innovative research ideas on how to solve problems using artificial intelligence, both in R&D and real-time applications are presented. Chapters describe the advanced prototypes, systems, methodologies, tools and techniques and general survey papers, which indicate future directions. These Chapters are extended papers from the Second Serbian International Conference on Applied Artificial Intelligence (SICAAI), which was held in Kragujevac, Serbia, on May 19-20, 2023.