The Microbiology of the Drop-in Biofuel Production is a comprehensive resource that provides basic and applied knowledge, technologies, and regulations of current drop-in biofuel production. This book focuses on liquid drop-in biofuel produced trough biochemical process and all the important aspect like renewable substrates, biochemical routes, genetic modifications, technology innovations, regulation, commercialization as well as the future perspectives to be implemented. Starting with an introductory chapter about the conventional methods to produce drop-in biofuels, the book will guide the reader into the specific biochemical processes for its production, covering aspect like renewable feedstocks, microorganism, classification, and the circular economy around all the production process. This book provides a detailed overview about the microbiology, regulation and commercialization aspects of drop-in biofuel production and is essential for researchers, students, and professors working in the bioenergy area.