The second edition of the textbook Enzymes- Catalysis, Kinetics, and Mechanisms focuses on the two broad mechanistic facets of enzymology namely, the chemical and the kinetic. It endeavors to bring out the synergy between enzyme structures and mechanisms. Written with a self-study approach in mind, the emphasis is on how to begin experiments with an enzyme and subsequently analyze the data. The book is divided into six major sections- 1) Enzyme Catalysis - A Perspective, 2) Enzyme Kinetic Practice and Measurements, 3) Elucidation of Kinetic Mechanisms, 4) Chemical Mechanisms and Catalysis, 5) Exploiting Enzymes, and 6) An end piece on Frontiers in Enzymology. The individual concepts are treated as stand-alone short sections. In case the reader needs to use any one concept, it should be possible with minimal cross-referencing to the rest of the book. Further, the book presents specialized techniques and complex approaches that require involved experimentation in theory with suitable references to guide the reader. The book is proposed more as a textbook in a self-learning mode to students of modern biology, particularly those with limited exposure to quantitative aspects and organic chemistry.