This two-volume set, LNCS 15612 and 15613 constitutes the refereed proceedings of the 28th European Conference on Applications of Evolutionary Computation, EvoApplications 2025, held as part of EvoStar 2025, in Trieste, Italy, during April 23-25, 2025, and co-located with the EvoStar events, EvoCOP, EvoMUSART, and EuroGP. The 50 full papers and 18 short papers presented in this book were carefully reviewed and selected from 104 submissions. These papers have been organized in the following topical sections: Part I: EvoApplications. Part II: Evolutionary machine learning; 30 years of particle swarm optimisation; Analysis of Evolutionary Computation Methods: Theory, Empirics, and Real-World Applications; Bio-inspired Algorithms for Green Computing and Sustainable Complex Systems; Computational Intelligence for Sustainability; EvoLLMs (Integrating Evolutionary Computing with Large Language Models (LLMs); Evolutionary Computation in Edge, Fog, and Cloud Computing; Evolutionary Computation in Image Analysis, Signal Processing, and Pattern Recognition; Machine Learning and AI in Digital Healthcare and Personalized Medicine; Soft Computing Applied to Games.