This book constitutes the refereed proceedings of the 16th International Conference on Verified Software, VSTTE 2024, held in Prague, Czech Republic, during October 14-15, 2024. The 6 full papers, as well as one invited paper, were carefully reviewed and selected for inclusion in the book. The conference received 6 submissions this year. The papers deal with the interaction of theory development, tool evolution and experimental validation in science and technology of software verification.