This volume LNCS 15557 constitutes the refereed proceedings of 16th International Conference on Intelligent Human Computer Interaction, IHCI 2024, held in Twente, The Netherlands, during November 13-16, 2024. The 37 full papers and 2 short papers were carefully reviewed and selected from 107 submissions. They were categorized under the topical sections as follows: HCI across domains Augmented & virtual reality Usability & UX Healthcare & clinical AI Centric HCI .