This book LNICST 622 constitutes the refereed proceedings of the Second EAI International Conference on Security and Privacy in Cyber-Physical Systems and Smart Vehicles, SmartSP 2024, held in New Orleans, LA, USA, during November 7-8, 2024. The 18 full papers were carefully reviewed an selected from 47 submissions. The proceedings focus on Emerging Applications, Hardware and Firmware Security, Adversarial Attacks in Autonomous Systems, Ethics, Privacy, Human-Centric Considerations and Security Techniques for Cyber-Physical Systems.