This book discusses validated in-vitro biotechnological interventions that have reshaped the landscape of plant genetic resource conservation. It covers essential topics such as collection processes, disease indexing, in-vitro culture establishment, multiplication techniques, and storage solutions ranging from short- to long-term strategies like cryopreservation. By addressing the challenges of ex-situ conservation management, this work offers a guide to preserving rare and endangered plants against the backdrop of climate change and unsustainable utilization. The chapters delve into critical themes such as slow growth strategies and synthetic seed technology for mid-term storage solutions. This book is for academicians, postgraduate students, and researchers in botany and plant biotechnology sectors, as well as molecular biologists and conservation enthusiasts.