The 8-volume set LNCS 16000-16008 constitutes the proceedings of the 45th Annual International Cryptology Conference, CRYPTO 2025, which took place in Santa Barbara, CA, USA, during August 17-21, 2025. The total of 156 full papers presented in the proceedings was carefully reviewed and selected from 643 submissions. They focus on cryptographic topics such as foundational theory and mathematics; the design, proposal, and analysis of cryptographic primitives and protocols; secure implementation and optimization in hardware or software; applied aspects of cryptography.