This book constitutes the refereed proceedings of the 7th International Conference on Information Technology in Bio- and Medical Informatics, ITBAM 2016, held in Porto, Portugal, in September 2016, in conjunction with DEXA 2016. The 9 revised long papers presented together with 11 poster papers were carefully reviewed and selected from 26 submissions. The papers address the following topics: biomedical data analysis and warehousing; information technologies in brain science; and social networks and process analysis in biomedicine.