This book constitutes the refereed proceedings of the 14th International Conference on Practical Applications of Scalable Multi-agent Systems, PAAMS 2016, held in Sevilla, Spain, in June 2016. The 9 revised full papers, 10 short papers, and 16 Demo papers were carefully reviewed and selected from 58 submissions (39 full paper and 19 Demo paper submissions. The papers report on the application and validation of agent-based models, methods, and technologies in a number of key application areas, including day life and real world, energy and networks, human and trust, markets and bids, models and tools, negotiation and conversation, scalability and resources. .