This book constitutes the thoroughly refereed post-workshop proceedings of the 16th International Workshop on Information Security Applications, WISA 2015, held on Jeju Island, Korea, in August 2015. The 35 revised full papers presented in this volume were carefully reviewed and selected from 78 submissions. The papers are organized in topical sections such as hardware security; cryptography, side channel attacks and countermeasures; security and threat analysis; IoT security; network security; cryptography; application security.