This book constitutes the refereed proceedings of the 16th International Conference on Ad-hoc, Mobile, and Wireless Networks, ADHOC-NOW 2017, held in Messina, Italy, in September 2017. The 22 full and 9 short papers presented in this volume were carefully reviewed and selected from 55 submissions. The contributions were organized in topical sections named: internet of things; security; smart city; ad-hoc networks; implementations and validations; wireless sensor networks; data management; wireless systems.