This book constitutes the refereed proceedings of the 27th Australasian Database Conference, ADC 2016, held in Sydney, NSW, Australia, in September 2016. The 33 full papers presented together with 11 demo papers were carefully reviewed and selected from 55 submissions. The mission of ADC is to share novel research solutions to problems of today's information society that fulfill the needs of heterogeneous applications and environments and to identify new issues and directions for future research. The topics of the presented papers are related to all practical and theoretical aspects of advanced database theory and applications, as well as case studies and implementation experiences.