This book constitutes the proceedings of the 20th International Conference on Theory and Practice of Digital Libraries, TPDL 2016, held in Hannover, Germany, in September 2016. The 28 full papers, 5 posters and 8 short papers presented in this volume were carefully reviewed and selected from 93 submissions. They were organized in topical sections named: Digital Library Design; User Aspects; Search; Web Archives; Semantics; Multimedia and Time Aspects; Digital Library Evaluation; Digital Humanities; e-Infrastructures.