This book constitutes the thoroughly refereed post-conference proceedings of the 9th International Joint Conference on Biomedical Engineering Systems and Technologies, BIOSTEC 2016, held in Rome, Italy, in February 2016. The 22 revised full papers presented were carefully reviewed and selected from a total of 321 submissions. The papers are organized in topical sections on biomedical electronics and devices; bioimaging; bioinformatics models, methods and algorithms; bio-inspired systems and signal processing; health informatics.