part, Section I: Introduction -- chapter 1 Introduction* -- part, Section II: History of Plant Tissue Culture -- chapter 2 History of Plant Tissue and Cell Culture -- part, Section III: Supporting Methodologies -- chapter 3 Getting Started with Tissue Culture: Media Preparation, Sterile Technique, and Laboratory Equipment -- chapter 4 Histological Techniques -- chapter 5 Photographic Methods for Plant Cell and Tissue Culture* -- chapter 6 Elements of In Vitro Research -- chapter 7 A Brief Introduction to Plant Anatomy -- chapter 8 Plant Growth Regulators in Plant Tissue Culture and Development -- chapter 9 Software and Databases as Tools for Analyzing Nucleic Acid and Protein Sequences* -- chapter 10 Molecular Approaches to the Study of Plant Development -- part, Section IV: Propagation and Development Concepts -- chapter 11 Shoot Culture Procedures -- chapter 12 Propagation from Nonmeristematic Tissues: Organogenesis -- chapter 13 Molecular Aspects of In Vitro Shoot Organogenesis -- chapter 14 Propagation from Nonmeristematic Tissues: Nonzygotic Embryogenesis* -- chapter 15 Some Developmental and Molecular Aspects of Somatic Embryogenesis (Nonzygotic Embryogenesis) -- part, Section V: Crop Improvement Techniques -- chapter 16 Use of Protoplasts for Plant Improvement -- chapter 17 Haploid Cultures -- chapter 18 Embryo Rescue -- chapter 19 Genetic Engineering Technologies* -- chapter 20 Genetically Modified Plant Controversies: Sensational Headlines versus Sensible Research -- chapter 21 Construction and Use of a Simple Gene Gun for Particle Bombardment* -- chapter 22 A Simple Illumination System for Visualizing Green Fluorescent Protein* -- chapter 23 Germplasm Preservation -- chapter 24 Valuable Secondary Products from In Vitro Culture -- part, Section VI: Special Topics -- chapter 25 Plant Pathology* -- chapter 26 Variation in Tissue Culture* -- chapter 27 Commercial Laboratory Production -- chapter 28 Indexing for Plant Pathogens -- chapter 29 Entrepreneurship for Biotechnology Ventures: From Bench to Bag.