chapter 1 Chemical Intermediates in Catalysis by Thiamine Diphosphate -- chapter 2 Mechanistic and Structural Studies on Thiamine Biosynthetic Enzymes -- chapter 3 Studies on the Structure and Function of Thiamine Pyrophosphokinase -- chapter 4 New Perspectives on the Cellular Role of Thiamine Triphosphate and Thiamine Triphosphatase -- chapter 5 How Thiamine Works in Enzymes: Time-Resolved NMR Snapshots of TDP-Dependent Enzymes in Action -- chapter 7 Ligand-Induced Conformational Changes in Thiamine Diphosphate�Dependent Enzymes: Comparison Between Crystal and Solution Structures -- chapter 8 Enantioselective Syntheses of Hydroxy Ketones via Benzoylformate Decarboxylase- and Benzaldehyde Lyase-Catalyzed C�C Bond Formation -- chapter 9 Benzoylformate Decarboxylase: Lessons in Enzymology -- chapter 10 New Concept on the Nature of the Induced Absorption Band of Holotransketolase -- chapter 11 Structure of the A-Carbanion/Enamine Reaction Intermediate in the Active Site of Transketolase, Determined by Kinetic Crystallography -- chapter 12 Yeast Pyruvate Decarboxylase: New Features of the Structure and Mechanism -- chapter 13 Solvent and Carbon Kinetic Isotope Effects on Active-Site and Regulatory-Site Variants of Yeast Pyruvate Decarboxylase -- chapter 14 Insights into the Mechanism and Regulation of Bacterial Acetohydroxyacid Synthases -- chapter 15 Structure and Properties of Acetohydroxyacid Synthase -- chapter 16 Exploring the Substrate Specificity of Benzoylformate Decarboxylase, Pyruvate Decarboxylase, and Benzaldehyde Lyase -- chapter 17 Benzoylformate Decarboxylase: Intermediates, Transition States, and Diversions -- chapter 18 Structural and Functional Organization of Pyruvate Dehydrogenase Complexes -- chapter 19 The Pyruvate Dehydrogenase Multienzyme Complex -- chapter 20 Activation and Transfer of Lipoic Acid in Protein Lipoylation in Mammals -- chapter 21 Central Organization of Mammalian Pyruvate Dehydrogenase (PD) Complex and Lipoyl Domain�Mediated Activated Function and Control of PD Kinases and Phosphatase 1 -- chapter 22 Physiological Effects of Replacing the PDH Complex of E. coli by Genetically Engineered Variants or by Pyruvate Oxidase -- chapter 23 Structure and Intersubunit Information Transfer in the E. coli Pyruvate Dehydrogenase Multienzyme Complex -- chapter 24 Structure, Function, and Regulation of Pyruvate Dehydrogenase Kinase -- chapter 25 Three-Dimensional Structures for Components and Domain of the Mammalian Branched-Chain a-Ketoacid Dehydrogenase Complex -- chapter 26 Variability of Human Pyruvate Dehydrogenase Complex Deficiency -- chapter 27 Kinetic Studies of Human Pyruvate Dehydrogenase and Its Mutants: Interactions with Thiamine Pyrophosphate -- chapter 28 The Complexity of Single-Gene Disorders: Lessons from Maple Syrup Urine Disease and Thiamine Responsiveness -- chapter 29 Thiamine Pyrophosphate: An Essential Cofactor in the Mammalian Metabolism of 3-Methyl-Branched Fatty Acids -- chapter 30 Pathogenesis of Selective Neuronal Loss in Wernicke�Korsakoff Syndrome: Role of Oxidative Stress -- chapter 31 Thiamine-Responsive Megaloblastic Anemia Syndrome: Clinical Aspects and Molecular Genetics -- chapter 32 Accomplishments and Future Directions.