1. Introduction / Kenneth E. Avis -- 2. Principles of large-scale cryopreservation of cells, microorganisms, protein solutions, and biological products / Richard Wisniewski -- 3. Large-scale cryopreservation : process development for freezing and thawing of large volumes of cell suspensions, protein solutions, and biological products / Richard Wisniewski -- 4. Method selection considerations for long-term preservation of mammalian cells and microorganisms / Carmen M. Wagner -- 5. Cryopreservation of mammalian cell cultures / William H. Siegel -- 6. Freeze-drying of human live virus vaccines / Steven S. Lee, Fangdong Yin -- 7. The design and use of thermal transport containers / Thomas C. Pringle.