chapter 1 Introduction / chapter 2 An Introduction to the Problems of Working with Trees / chapter 3 Wood Microscopical Techniques / chapter 4 Conventional (Chemical-?xation) Transmission Electron Microscopy and Cytochemistry of Angiosperm Trees / chapter 5 Chemical and Cryo-?xation for Transmission Electron Microscopy of Gymnosperm Cambial -- Cells Kim H. Rensing / chapter 6 Deep-etching Electron Microscopy and 3-dimensional Cell Wall Architecture / chapter 7 Secondary Ion Mass Spectrometry Microscopy: Application to the Analysis of Woody Samples / chapter 8 Immunolocalisation of the Cytoskeleton in the Secondary Vascular System of Angiosperm Trees and its Visualisation Using Epi?uorescene Microscopy / chapter 9 Immunolocalisation and Visualisation of the Cytoskeleton in Gymnosperms using Confocal Laser Scanning Microscopy / chapter 10 Cell Walls of Woody Plants: Autoradiography and Ultraviolet Microscopy / chapter 11 Cell Walls of Woody Tissues: Cytochemical, Biochemical and Molecular Analysis of Pectins and Pectin Methylesterases -- Fabienne Micheli, Fabienne E. Ermel, Marianne Bordenave, Luc Richard and Ren�e Goldberg / chapter 12 Immunolocalisation of Enzymes of Lignification / chapter 13 Sampling of Cambial Region Tissues for High Resolution Analysis / chapter 14 Biochemistry and Quantitative Histochemistry of Wood / chapter 15 Protein Analysis in Perennial Tissues / chapter 16 The Use of GUS Histochemistry to Visualise Lignification Gene Expression In Situ during Wood Formation -- Simon Hawkins, Gilles Pilate, Eric Duverger, Alain Michel Boudet and Jacqueline Grima-Pettenati / chapter 17 High Resolution In Situ Hybridisation in Woody Tissues / chapter 18 Random Amplification of Polymorphic DNA and Reverse Transcription Polymerase Chain Reaction of RNA in Studies of Sapwood and Heartwood