chapter 1 Concepts for Protein Engineering / chapter 2 Sequence Families and Modular Organization of Carbohydrate-Active Enzymes -- chapter 3 Analyzing Three-Dimensional Structures of Variant Enzymes / chapter 4 Quantitative Modeling of Lipase Enantioselectivity / chapter 5 Rational Redesign of Haloalkane Dehalogenases Guided by Comparative Binding Energy Analysis -- chapter 6 Computer Simulations: A Tool for Investigating the Function of Complex Biological Macromolecules -- chapter 7 Calculations of Ionization Equilibria in Proteins / chapter 8 Modeling and Optimization of Directed Evolution Protocols / chapter 9 Rational Redesign of Enzymes / chapter 10 Details in the Reaction Mechanism of Chitinases -- chapter 11 Kinetic Evolution to the Catalytic Core of the Bacterial Phosphotriesterase / chapter 12 Protein Engineering of PQQ Glucose Dehydrogenase / chapter 13 The Profiline Rule: A Concept for Engineering Protein Stability / chapter 14 Homing Endonucleases: Tools and Targets for Protein Engineering -- chapter 15 Evolutionary Methods for Protein Engineering / chapter 16 Directed Evolution by Random Mutagenesis: A Critical Evaluation / chapter 17 Enzyme Engineering by Phage Display / chapter 18 In Vivo Gene Shuffling in Yeast: A Fast and Easy Method for Directed Evolution of Enzymes -- chapter 19 Effective DNA Shuffling Methods for Enzyme Evolution / chapter 20 Exploring the Functional Space of Combinatorial Mutant Libraries for the Directed Evolution of Novel Enzyme Activities -- chapter 21 Modifying the Character of an Enzyme by Producing Chimeric Enzymes: Chimeric -glucosidases as an Illustration -- chapter 22 Assay Systems for Screening or Selection of Biocatalysts / chapter 23 Screening of Enzyme Variants for Thermostability / chapter 24 Combinatorial Mutagenesis Algorithms, Digital Imaging Spectroscopy, and Solid-Phase Assays for Directed Evolution -- chapter 25 Screen Automation and Robotics / chapter 26 Screening for Enantioselective Enzymes / chapter 27 Enzyme Engineering by Microbial Cell Surface Display / chapter 28 Overexpression and Secretion of Biocatalysts in Pseudomonas / chapter 29 Analysis of Catalytic and Structural Stability of Native and Covalently Modified Enzymes.