Establishing and Maintaining an IVF Laboratory--Setting up an ART laboratory; Jacques Cohen, Mina Alikani, Antonia Gilligan, and Tim Schimmel--Quality control: Maintaining stability in the laboratory; David H. McCulloh--The ART laboratory: Current standards; Cecilia Sjblom--Gamete Collection, Preparation and Selection--Evaluation of sperm; Kaylen M. Silverberg and Tom Turner--Sperm preparation techniques; Harold Bourne, Janell Archer, David H. Edgar, and H. W. Gordon Baker--Sperm chromatin assessment; Ashok Agarwal, Igor Tsarev, Juris Erenpreiss, and Rakesh Sharma--Oocyte retrieval and selection; Laura F. Rienzi and Filippo M. Ubaldi --Preparation and evaluation of oocytes for ICSI; Irit Granot and Nava Dekel--Hyaluronic acid binding-mediated sperm selection for ICSI; Gabor Huszar--Intracytoplasmic morphologically selected sperm injection; Monica Antinori--Use of in vitro maturation in a clinical setting: Patient populations and outcomes; Yoshiharu Morimoto, Aisaku Fukuda, and Manabu Satoh--Micromanipulation--Micromanipulation; Frank L. Barnes--Intracytoplasmic sperm injection: Technical aspects; Queenie V. Neri, Devin Monahan, Zev Rosenwaks, and Gianpiero D. Palermo--Assisted hatching; Anna Veiga and Itziar Belil--Human embryo biopsy procedures; Alan R. Thornhill, Christian Ottolini, and Alan H. Handyside--Culture, Selection and Transfer of the Human Embryo--Analysis of fertilization; Thomas Ebner--Culture systems for the human embryo; David K. Gardner and Michelle Lane--Evaluation of embryo quality: Analysis of morphology and quantification of nutrient utilization and the metabolome; Denny Sakkas and David K. Gardner--Evaluation of embryo quality: Time-lapse imaging to assess embryo morphokinesis; Natalia Basile, Juan Garca-Velasco, and Marcos Meseguer--Evaluation of embryo quality: Proteomic strategies; Mandy Katz-Jaffe--Cryopreservation--The human oocyte: Controlled rate cooling; Andrea Borini and Veronica Bianchi--The human oocyte: Vitrification; Masashige Kuwayama--The human embryo: Slow freezing; Nikica Zaninovic, Richard Bodine, Robert N. Clarke, Sam Jones, Ye Zhen, and Lucinda L. Veeck Gosden--The human embryo: Vitrification; Zsolt Peter Nagy, Ching-Chien Chang, and Gbor Vajta--Diagnosis of Genetic Disease in Preimplantation Embryos--Severe male factor: Genetic consequences and recommendations for genetic testing; Willy Lissens and Katrien Stouffs; Polar body biopsy and its clinical application; Markus Montag--Preimplantation genetic diagnosis for infertility; Dagan Wells and Elpida Fragouli--Genetic analysis of the embryo; Yuval Yaron, Veronica Gold, Sagit Peleg-Schalka, and Mira Malcov--Implantation--The analysis of endometrial receptivity; Tamara Garrido-Gomez, Francisco Domnguez, Maria Ruiz, Felip Vilella, and Carlos Simon--Future Directions and Clinical Applications--Human embryonic stem cells; Rachel Eiges, Michal Avitzour, and Benjamin Reubinoff--Microfluidics in ART: Current progress and future directions; Jason E. Swain, Thomas B. Pool, Shuichi Takayama, and Gary D. Smith--Index.