part Section 1: Introduction, Microscopy, Fluorophores -- chapter 1 Fluorescence Lifetime-Resolved Image: What, Why, How-A Profilogue / chapter 2 Principles of Fluorescence for Quantitative Fluorescence Microscopy / chapter 3 Visible Fluorescent Proteins for FRET-FLIM / part Section 2: Instrumentation -- chapter 4 Wide-Field Fluorescence Lifetime Imaging Mircoscopy Using a Gated Image Intensifier Camera / chapter 5 Frequency-Domain FILM / chapter 6 Laser Scanning Confocal FLIM Microscopy / chapter 7 Multiphoton Fluorescence Lifetime Imaging at the Dawn of Clinical Application / chapter 8 FLIM Microscopy with a Streak Camera: Monitoring Metabolic Processes in Living Cells and Tissues / chapter 9 Spectrally Resolved Fluorescence Lifetime Imaging Microscopy / chapter 10 Time-Resloved Fluorescence Anisotropy / part Section 3: Data Analysis -- chapter 11 General Concerns of FLIM Data Representation and Analysis: Frequency-Domain Model-Free Analysis -- chapter 12 Nonlinear Curve-Fitting Methods for Time-Resolved Data Analysis -- chapter 13 Global Analysis of Frequency Domain FLIM Data / part Section 4: Applications -- chapter 14 FLIM Applications in the Biomedical Sciences