Başlık:
The condensed protocols from molecular cloning : a laboratory manual
Yazar:
Sambrook, Joseph.
ISBN:
9780879697723
9780879697716
Yayın Bilgileri:
Cold Spring Harbor, N.Y. : Cold Spring Harbor Laboratory Press, c2006.
İçerik:
Plasmids and their usefulness in molecular cloning -- Bacteriophage and its vectors -- Working with bacteriophage M13 -- Working with high-capacity vectors -- Gas electrophoresisof DNA and pulsed-field agarose -- Preparation and analysis of eukaryotic genomic DNA -- Extraction, purification, and analysis of mRNA from eukaryotic cells -- In vitro amplification of DNA by the polymerase chain reaction -- Preparation of radiolabeled DNA and RNA probes -- Working with synthetic oligonucleotide probes -- Preparation of cDNA libraries and gene identification -- DNA sequencing -- Mutagenesis -- Screening expression libraries -- Expression of cloned genes in escherichia coli -- Introducing cloned genes into cultured mammalian cells -- Analysis of gene expression in cultured mammalian cells -- Protein interaction technologies.
Yazar Ek Girişi:
Kopya:
Rafta:*
Kütüphane | Materyal Türü | Demirbaş Numarası | Yer Numarası | Durumu/İade Tarihi | Materyal Ayırtma |
---|---|---|---|---|---|
Arıyor... | Kitap | 7.2/12/397734 | QH 440.5 S187 2006 | Arıyor... | Arıyor... |