This book provides a comprehensive and concise overview of the bioimaging tools used in preclinical research in life sciences. It is divided into three sections: Fundamentals and Applications of Bioimaging Tools in Preclinical Research, Functional, Quantitative, and Advanced Multimodal Approaches in Preclinical Bioimaging, and Emerging Technologies and Computational Advances in Bioimaging. While the first two sections cover fundamentals and preclinical applications of the bioimaging modalities, the third section focuses on emerging technologies including the use of artificial intelligence in bioimaging. Aiming to provide an concise overview of bioimaging modalities, this book will be an essential resource for researchers in the field of life sciences including bioengineering, tissue engineering, and regenerative medicine. Chapter "Quantifying Biological Processes in Motion and Across Scales" is available open access under a Creative Commons Attribution 4.0 International License via link.springer.com.